Professor of Cell & Molecular Biology
Office: BSB 2.03.44
Phone: (210) 458-4507
John.McCarrey@utsa.edu
Research Interests
Research in my laboratory is centered on the development, differentiation, and manipulation of mammalian germ cells - the cells that form the gemetes (sperm in males and eggs in females). Our primary experimental system is the mouse, however we also conduct studies in baboons, opossums, and other mammalian species. We are interested in 1) differential gene expression in germ cells and the mechanisms that regulate this, 2) X-chromasome activity and inactivity in germ cells, 3) genomic imprinting and how this becomes established during gematogenesis, 4) animal cloning and abnormalities this proces may induce in genetic and epigenetic programing mechanisms, and 5) manipulation of germ cells from baboons to generate transgenic non-human primates.
Recent Publications
Davidow LS, Breen M, Duke SE, Samollow PB, McCarrey JR, Lee JT. The search for a marsupial XIC reveals a break with vertebrate synteny. Chromosome Res. 2007;15(2):137-46.
Liu D, Brockman JM, Dass B, Hutchins LN, Singh P, McCarrey JR, MacDonald CC, Graber JH. Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis. Nucleic Acids Res. 2007;35(1):234-46.
Namekawa SH, Park PJ, Zhang LF, Shima JE, McCarrey JR, Griswold MD, Lee JT. Postmeiotic sex chromatin in the male germline of mice. Curr Biol. 2006 Apr 4;16(7):660-7.
McCarrey JR, Geyer CB, Yoshioka H. Epigenetic regulation of testis-specific gene expression. Ann N Y Acad Sci. 2005 Dec;1061:226-42.
Dudley CJ, Hubbard GB, Moore CM, Dunn BG, Raveendran M, Rogers J, Nathanielsz PW, McCarrey JR, Schlabritz-Loutsevitch NE. A male baboon (Papio hamadryas) with a mosaic 43,XXY/42,XY karyotype. Am J Med Genet A. 2006 Jan 1;140(1):94-7.
Wang PJ, Page DC, McCarrey JR. Differential expression of sex-linked and autosomal germ-cell-specific genes during spermatogenesis in the mouse. Hum Mol Genet. 2005 Oct 1;14(19):2911-8.
Yamazaki Y, Low EW, Marikawa Y, Iwahashi K, Bartolomei MS, McCarrey JR, Yanagimachi R. Adult mice cloned from migrating primordial germ cells. Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11361-6.
Xu G, Spivak G, Mitchell DL, Mori T, McCarrey JR, McMahan CA, Walter RB, Hanawalt PC, Walter CA. Nucleotide excision repair activity varies among murine spermatogenic cell types. Biol Reprod. 2005 Jul;73(1):123-30.
Thomas K, Sung DY, Yang J, Johnson K, Thompson W, Millette C, McCarrey J, Breitberg A, Gibbs R, Walker W. Identification, characterization, and functional analysis of sp1 transcript variants expressed in germ cells during mouse spermatogenesis. Biol Reprod. 2005 Apr;72(4):898-907.
Huamani J, McMahan CA, Herbert DC, Reddick R, McCarrey JR, MacInnes MI, Chen DJ, Walter CA. Spontaneous mutagenesis is enhanced in Apex heterozygous mice. Mol Cell Biol. 2004 Sep;24(18):8145-53.
Geyer CB, Kiefer CM, Yang TP, McCarrey JR. Ontogeny of a demethylation domain and its relationship to activation of tissue-specific transcription. Biol Reprod. 2004 Sep;71(3):837-44.
Walter CA, Intano GW, McMahan CA, Kelner K, McCarrey JR, Walter RB. Mutation spectral changes in spermatogenic cells obtained from old mice. DNA Repair (Amst). 2004 May 4;3(5):495-504.
Shima JE, McLean DJ, McCarrey JR, Griswold MD. The murine testicular transcriptome: characterizing gene expression in the testis during the progression of spermatogenesis. Biol Reprod. 2004 Jul;71(1):319-30.
Yamazaki Y, Mann MR, Lee SS, Marh J, McCarrey JR, Yanagimachi R, Bartolomei MS. Reprogramming of primordial germ cells begins before migration into the genital ridge, making these cells inadequate donors for reproductive cloning. Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12207-12.
Ding F, Patel C, Ratnam S, McCarrey JR, Chaillet JR. Conservation of Dnmt1o cytosine methyltransferase in the marsupial Monodelphis domestica. Genesis. 2003 Aug;36(4):209-13.
Weissig H, Narisawa S, Sikstrom C, Olsson PG, McCarrey JR, Tsonis PA, Del Rio-Tsonis K, Millan JL. Three novel spermatogenesis-specific zinc finger genes. FEBS Lett. 2003 Jul 17;547(1-3):61-8.
Walter CA, Walter RB, McCarrey JR. Germline genomes--a biological fountain of youth? Sci Aging Knowledge Environ. 2003 Feb 26;2003(8):PE4.
McCarrey JR, Watson C, Atencio J, Ostermeier GC, Marahrens Y, Jaenisch R, Krawetz SA. X-chromosome inactivation during spermatogenesis is regulated by an Xist/Tsix-independent mechanism in the mouse. Genesis. 2002 Dec;34(4):257-66.
Chan J, Robinson ES, Yeh IT, McCarrey JR. Absence of ras gene mutations in UV-induced malignant melanomas correlates with a dermal origin of melanocytes in Monodelphis domestica. Cancer Lett. 2002 Oct 8;184(1):73-80.
Intano GW, McMahan CA, McCarrey JR, Walter RB, McKenna AE, Matsumoto Y, MacInnes MA, Chen DJ, Walter CA. Base excision repair is limited by different proteins in male germ cell nuclear extracts prepared from young and old mice. Mol Cell Biol. 2002 Apr;22(7):2410-8.