James P. Chambers, Ph.D.
Professor of Biochemistry
Office: BSE 3.224
Phone: (210) 458-5663
James.Chambers@utsa.edu
Research Interests
Regulation of intracellular free calcium and its maintenance at extremely low concentrations (submicromolar) is one of the main tasks of cells. Transient calcium fluxes induce the activation of calcium-dependent second messenger systems. Thus, extrusion of excess calcium is critical to the well-being of the cell. Bulk amounts of calcium are removed from the cell cytosol by a Na+/Ca2+ exchanger. In contrast, removal of threshold amounts of free calcium is dependent upon a low capacity, high affinity plasma membrane calcium pump that couples Ca2+ translocation with ATP hydrolysis. We are interested in the characterization of calcium binding proteins in the cell and in particular the kinetic analysis of the [Ca2+ + MG 2+] - dependent adenosine-triphosphatase.
Recent Publications
Murthy AK, Chambers JP, Meier PA, Zhong G, Arulanandam BP. Intranasal vaccination with a secreted chlamydial protein enhances resolution of genital Chlamydia muridarum infection, protects against oviduct pathology, and is highly dependent upon endogenous gamma interferon production. Infect Immun. 2007 Feb;75(2):666-76.
Daum LT, Canas LC, Klimov AI, Shaw MW, Gibbons RV, Shrestha SK, Myint KS, Acharya RP, Rimal N, Reese F, Niemeyer DM, Arulanandam BP, Chambers JP. Molecular analysis of isolates from influenza B outbreaks in the U.S. and Nepal, 2005. Arch Virol. 2006 Sep;151(9):1863-74.
Daum LT, Shaw MW, Klimov AI, Canas LC, Macias EA, Niemeyer D, Chambers JP, Renthal R, Shrestha SK, Acharya RP, Huzdar SP, Rimal N, Myint KS, Gould P. Related Articles, Links Free Full Text Influenza A (H3N2) outbreak, Nepal. Emerg Infect Dis. 2005 Aug;11(8):1186-91.
Daum LT, Ye K, Chambers JP, Santiago J, Hickman JR, Barnes WJ, Kruzelock RP, Atchley DH. Related Articles, Links Abstract Comparison of TaqMan and Epoch Dark Quenchers during real-time reverse transcription PCR. Mol Cell Probes. 2004 Jun;18(3):207-9.