Dr. Karl Klose
My lab is interested in bacterial pathogenesis -- how bacteria cause disease. I have worked most extensively with Vibrio cholerae, the bacterium that causes cholera, and I am also researching Francisella tularensis, the bacterium that causes tularemia, or rabbit fever.
Cholera is found only where there are widespread problems with sanitation, so improving water and food supplies would eliminate the disease. Since that is unlikely to occur, a safe, cheap, effective vaccine is needed that would protect people. To design such a vaccine, my lab is addressing questions such as: How does V. cholerae know that it is in a human body and that that is the place to express genes necessary for its survival and disease potential? What are the genetic factors responsible for V. cholerae to cause disease? How does this organism persist in aquatic environments, which lead to human infection?
Very little is known about F. tularensis or about tularemia. It is a highly virulent organism and can easily be aerosolized, so it is classified by the Centers for Disease Control (CDC) as a Category A select agent with the highest potential to be used as a biological weapon. My lab is working to identify genetic factors responsible for F. tularensis to cause disease and to develop suitable vaccine candidates to protect against tularemia infection. My work in this area will be greatly enhanced by the completion of the UTSA high-level biocontainment (BSL-3) laboratory.
Book or Segment of Book - Segment of Book - Chapter - Edited Book
Syed, K. A. and Klose, K. E. “Vibrio cholerae flagellar synthesis and virulence” (pgs. 203-212). in Epidemiological and Molecular Aspects on Cholera (Infectious Disease). 2010. Eds. Ramamurthy, T. and Bhattacharya, S. K. Springer (New York).
Journal Publications - Journal Article - Article in Proceedings
Chatterjee, S., Ghosh, K., Raychoudhuri, A., Chowdhury, G., Bhattacharya, M. K., Mukhopadhyay, A. K., Ramamurthy, T., Bhattacharya, S. K., Klose, K. E., Nandy, R. K. 2009. Association of non-O1, non-O139 Vibrio cholerae among hospitalized diarrheal patients at Kolkata: a study on incidence, virulence factors and clonality among clinical strains. J. Clin. Microbiol. 47:1087-1095
Sorci, L., Martynowksi, D. Rodionov, D. A., Eyobo, Y., Zogaj, X., Klose, K. E., Nikolaev, E. V., Magni, G., Zhang, H., Osterman, A. L. 2009. Nicotinamide mononucleotide synthetase is the key enzyme for an alternative route of NAD biosynthesis in Francisella tularensis. Proc. Natl. Acad. Sci. USA. 106:3083-3088
Ray, H. J., Cong, Y., Murthy, A. K., Selby, D. M., Klose, K. E., Barker, J. R., Guentzel, M. N, Arulanandam, B. P. 2009. Oral Live Vaccine Strain Induced Protective Immunity Against Pulmonary Francisella tularensis Mediated by CD4+ T cells and Antibodies Including IgA. Clin. Vaccine Immunol. 16:444-452
Rodriguez S. A., Davis G, Klose K. E. 2009. Targeted gene disruption in Francisella tularensis by group II introns. Methods. 49:270-274.
Cong Y, Yu JJ, Guentzel MN, Berton MT, Seshu J, Klose KE, Arulanandam BP. 2009. Vaccination with a defined Francisella tularensis subsp. novicida pathogenicity island mutant (DeltaiglB) induces protective immunity against homotypic and heterotypic challenge. Vaccine 27:5554-5561.
Syed KA, Beyhan S, Correa N, Queen J, Liu J, Peng F, Satchell KF, Yildiz F, Klose K. E. 2009. The Vibrio cholerae flagellar regulatory hierarchy controls expression of virulence factors. J. Bacteriol. 191:6555-6570.
Moisi M, Jenul C, Butler SM, New A, Tutz S, Reidl J, Klose KE, Camilli A, Schild S. 2009. A novel regulatory protein involved in motility of Vibrio cholerae. J. Bacteriol. 191:7027-7038.
Journal Publications -
Weber GG, Klose KE. 2011. The complexity of ToxT-dependent transcription in Vibrio cholerae.. Indian J. Med Res. 133(2):201-206.
Murthy AK, Chaganty BK, Troutman T, Guentzel MN, Yu JJ, Ali SK, Lauriano CM, Chambers JP, Klose KE, Arulanandam BP. 2011. Mannose-containing oligosaccharides of non-specific human secretory immunoglobulin A mediate inhibition of Vibrio cholerae biofilm formation. PLoS One. 6(2):e16847.
Chu P, Rodriguez AR, Arulanandam BP, Klose KE. 2011. Tryptophan prototrophy contributes to Francisella tularensis evasion of gamma interferon-mediated host defense. Infect Immun. 79(6):2356-2361.
Nallaparaju KC, Yu JJ, Rodriguez SA, Zogaj X, Manam S, Guentzel MN, Seshu J, Murthy AK, Chambers JP, Klose KE, Arulanandam BP. 2011. Evasion of IFN-γ signaling by Francisella novicida is dependent upon Francisella outer membrane protein C. PLoS One. 6(3):e18201.
Childers BM, Cao X, Weber GG, Demeler B, Hart PJ, Klose KE. 2011. N-terminal residues of the Vibrio cholerae virulence regulatory protein ToxT involved in dimerization and modulation by fatty acids. J Biol Chem. 286(32):28644-28655.
Hankins JV, Madsen JA, Giles DK, Childers BM, Klose KE, Brodbelt JS, Trent MS. 2011. Elucidation of a novel Vibrio cholerae lipid A secondary hydroxy-acyltransferase and its role in innate immune recognition. Mol Microbiol. 81(5):1313-1329