Dr. Jose Lopez-Ribot
My laboratory studies the opportunistic pathogenic fungus Candida albicans. C. albicans is part of the normal human microbiota. However, as an opportunistic pathogen it is capable of causing overt disease (candidiasis), but usually only in hosts with defective immunity. The frequency of candidiasis has increased dramatically in the last decades as a result of an expanding population of immunocompromised patients. As a result, candidiasis is now the fourth most common nosocomial infections. The seriousness of this problem is heightened by the fact that, even with treatment using available antifungal agents, mortality rates lie in the 30- 40% range for these infected patients. As an opportunistic pathogen, it is clear that mechanisms of host immunity and pathogen virulence intertwine, giving rise to the highly complex nature of host-fungus interactions. However, the interplay between host immunity and fungal virulence has traditionally been ignored and most investigations into these topics are overwhelmingly “one-sided” which has resulted in a dangerous dichotomy between “microorganism-centered” and “host-centered” views of candidal pathogenesis. Thus, studies in my laboratory try to integrate these two facets to better understand and offer a more global perspective of C. albicans pathogenesis. Results have already provided new paradigms in the host-fungus relationship during candidiasis.
Book or Segment of Book
Sardi, J.C.O., I.T.A. Peixoto, A.Rodriguez-García, , L.J. Galan Wong, K. Arévalo-Niño, J.F. Höfling, R.B. Gonçalves-Reginaldo, C.G. Pierce, and J.L. Lopez-Ribot. 2010. Effects of plants used in Mexican traditional medicine on Candida albicans biofilm formation. In Microorganisms in Industry and Environment: From Scientific and Industrial Research to Consumer Products (Antonio Mendez-Vilas, ed.). World Scientific Publishing Co., Singapore.
Peixoto, I.T.A., J.C.O. Sardi, J.F. Höfling , R.B. Gonçalves, C.G. Pierce, J.L. Lopez-Ribot, A.Rodriguez-García, L.J. Galan-Wong, K. Arévalo-Niño. 2010. In vitro tests of polymers with extracts from plants used in Mexican traditional medicine on Candida albicans biofilm formation. In Microorganisms in Industry and Environment: From Scientific and Industrial Research to Consumer Products (Antonio Mendez-Vilas, ed.). World Scientific Publishing Co., Singapore.
Pierce, C.G., P. Uppuluri, and J.L. Lopez-Ribot. 2011. A method for the formation of Candida biofilms in 96 well microtiter plates and its application to antifungal susceptibility testing of biofilm cells. In “Laboratory Manual on Fungi” (V. Gupta, ed.). Springer. In press.
Journal Publications -
Srinivasan, A., P. Uppuluri, J.L. Lopez-Ribot and A.K. Ramasubramanian. 2011. Development of a High-Throughput Candida albicans Biofilm Chip PloS One. 6(4):e19036
Uppuluri, P., A. Srinivasan, A.K. Ramasubramanian and J.L. Lopez-Ribot. 2011. Effect of fluconazole, amphotericin B and caspofungin against Candida albicans biofilms under conditions of flow and on biofilm dispersion. Antimicrob. Agents Chemother. 55:3591-3
Chaturvedi, A.L. Lazzell, S. P. Saville, F. L. Wormley, C. Monteagudo and J. L. Lopez-Ribot. 2011. Validation of the tetracycline regulatable gene expression system for the study of the pathogenesis of infectious disease. PLoS One. 6(5): e20449
Robbins, N., P. Uppuluri, J. Nett, R. Rajendran, G. Ramage, J.L. Lopez-Ribot, D. Andes, and L. E. Cowen. 2011. Hsp90 Governs Dispersion and Drug Resistance of Fungal Biofilms. PLoS Pathogens. 7(9): e1002257. doi:10.1371/journal.ppat.1002257
Martins, M., A. L. Lazzell, J.L. Lopez-Ribot, M. Henriques, R. Oliveira. 2011. Effect of Exogenous Administration of Candida albicans Autoregulatory Alcohols in a Murine Model of Hematogenously Disseminated Candidiasis. Journal of Basic Microbiology. E-pub ahead of print
Chambers, J.P., J.-J. Yu, M. Jupelli, J.L Lopez-Ribot, J. J. Valdes and B.P. Arulanandam. 2011. Alpha-1 antitrypsin is markedly decreased following pulmonary F. tularensis challenge. Frontiers in Cellular and Infection Microbiology. 1.
Martins, M., M.H. Henriques, J.L. Lopez-Ribot and R.O. Oliveira. Addition of DNase Improves the In Vitro Activity of Antifungal Drugs against Candida albicans Biofilms. Mycoses. In press.
Dahlan, A.A., C. Haveman, G. Ramage. S. Redding and J.L. Lopez-Ribot. 2011. Sodium hypochlorite, chlorhexidine gluconate, and commercial denture cleansers as disinfecting agents against Candida albicans, an in vitro comparison study. General Dentistry. In press.
Martins, M., P. Uppuluri, D.P. Thomas, I. Cleary, M.H. Henriques, J.L. Lopez-Ribot and R.O. Oliveira. 2010. Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms. Mycopathologia. 169:323-31.
Uppuluri, P., A.K. Chaturvedi, A. Srinivasan, M. Banerjee, A.K. Ramasubramaniam, J.R. Köhler, D. Kadosh and J.L. Lopez Ribot. 2010. Dispersion as an Important Step in the Candida albicans Biofilm Developmental Cycle. PLoS Pathogens.6:e1000828.
Uppuluri, P., C.G. Pierce, D.P. Thomas, S.S. Bubeck, S.P. Saville and J.L. Lopez-Ribot. 2010. The transcriptional regulator Nrg1p controls Candida albicans biofilm formation and dispersion. Eukaryot. Cell 9:1531-7.
Dahlan, A.A., C. Haveman, G. Ramage. S. Redding and J.L. Lopez-Ribot. 2010. Sodium hypochlorite, chlorhexidine gluconate, and commercial denture cleansers as disinfecting agents against Candida albicans, an in vitro comparison study. General Dentistry.
Uppuluri, P., and J.L. Lopez-Ribot. 2010. An easy and economical in vitro method for the formation of Candida albicans biofilms under continuous conditions of flow. Virulence. 1(6):
Pierce, C.G., P. Uppuluri, S. Tummala, and J.L. Lopez-Ribot. 2010. A 96 well microtiter plate-based method for monitoring formation and antifungal susceptibility testing of Candida albicans biofilms. Journal of Visualized Experiments. 44: doi: 10.3791/2287.