Dr. Brian Hermann
Our laboratory studies the basic biology of spermatogonial stem cells (SSCs), which are adult-tissue stem cells responsible for sperm production in the mammalian testis and which are essential for male fertility. Ongoing studies in the lab are focused on 1) determining how these cells are regulated and behave like stem cells, 2) how we can prevent SSC loss due to sterilizing treatments, 3) how SSCs participate in the etiology of male infertility, and 4) how the male germline ages. Our work has potential implications for basic stem cell biology, reproduction, as well as translational significance for treatment and prevention of male infertility. The approaches we use in the lab are multidisciplinary, including animal models (rodents, primates), human tissues, an in vitro SSC culture system, an in vivo transplantation assay for stem cells, various wide-field microscopy techniques, as well as conventional and high-throughput molecular biology and genomic approaches.
Lab website: http://hermannlab.utsa.edu
Hermann, B.P., Sukhwani, M., Salati, J., Chu, T., Sheng, Y., and Orwig, K.E. 2011. “Separating spermatogonia from cancer cells in contaminated prepubertal primate testis cell suspensions.” Human Reproduction. 26(12):3222-31.
Hermann, B.P., Sukhwani, M., Winkler, F., Pascarella, J., Peters, K., Sheng, Y., Valli, H., Rodriguez, M., Ezzelarab, M., Dargo, G., Peterson, K., Masterson, K., Ramsey, C., Ward, T., Lienesch, M., Volk, A., Cooper, D., Thomson, A., Kiss, J.E., Penedo, C., Schatten, J.P., Mitalipov, S., and Orwig, K.E. 2011. "Spermatogonial stem cell transplantation into Rhesus testes regenerates spermatogenesis producing functional sperm." Cell Stem Cell. Submitted.
Hermann, B.P., Sukhwani, M., Hansel M.C., and Orwig, K.E. 2010. “Spermatogonial stem cells in higher primates: are there differences to those in rodents?” Reproduction 139:479-493.
Ben-Yehudah A., Easley C.A. 4th, Hermann B.P., Castro C., Simerly C., Orwig K.E., Mitalipov S., Schatten G. 2010 “Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns.” Stem Cell Res Ther. 1:24.
Hermann, B.P., Sukhwani, M., Simorangkir, D., Plant, T., and Orwig, K.E. 2009. “Molecular dissection of the male germ cell lineage identifies putative spermatogonial stem cells in rhesus macaques.” Human Reproduction. 24(7):1704-16.
Hermann, B.P., Hornbaker, K., Rice, D.A., Sawadogo, M., and Heckert, L.L. 2008. “In vivo regulation of FSH-receptor (Fshr) by the transcription factors USF1 and USF2 is cell specific.” Endocrinology 149(10):5297-306.
Hermann, B.P., Sukhwani, M., Lin, C., Sheng, Y., Tomko, J., Rodriguez, M., Shuttleworth, J., McFarland, D., Hobbs, R.M., Pandolfi, P.P., Schatten, G.P., and Orwig, K.E. 2007. “Characterization, cryopreservation, and ablation of spermatogonial stem cells in adult rhesus macaques.” Stem Cells 25(9):2330-2338.
Hermann, B.P., Hornbaker, K., Manimaran, R.R., and Heckert, L.L. 2007. “Distal regulatory elements are required for Fshr expression, in vivo.” Molecular and Cellular Endocrinology 260-262: 49-58.
Hermann, B.P. and Heckert, L.L. 2007. “ Transcriptional regulation of the FSH receptor: new perspectives.” Molecular and Cellular Endocrinology 260-262:100-108.